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1994-06-25
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Document 0645
DOCN M9460645
TI Diagnosis and differentiation of HIV-1 and HIV-2 infection by two rapid
assays in Nigeria.
DT 9408
AU Kline RL; Dada A; Blattner W; Quinn TC; Laboratory of Immunoregulation,
National Institute of Allergy and; Infectious Diseases, National
Institutes of Health, Bethesda,; Maryland.
SO J Acquir Immune Defic Syndr. 1994 Jun;7(6):623-6. Unique Identifier :
AIDSLINE MED/94231468
AB We evaluated the reliability, sensitivity, and specificity of two rapid
assays, TestPack HIV-1/HIV-2 and Genie HIV-1/HIV-2, in Lagos, Nigeria.
An alternative algorithm to EIA and Western blot was then examined with
the TestPack HIV-1/HIV-2 used as the screening test and the Genie
HIV-1/HIV-2 used as the supplemental test to differentiate HIV-1 and
HIV-2 infection. In all, 845 prostitutes were evaluated for HIV-1 and
HIV-2 infection using one of the two rapid tests and compared to EIA and
Western blot results. Of these 845 cases, 437 samples were analyzed by
both assays. Overall, 109 (12.7%) prostitutes were antibody positive for
HIV-1, 13 (1.5%) for HIV-2, and six (0.7%) were dually reactive for both
HIV-1 and HIV-2. Compared to Western blot, the Genie HIV-1/HIV-2 had a
slightly higher sensitivity and specificity (98.4% and 99.7%) than the
TestPack HIV-1/HIV-2 (97.6% and 99.3%). The alternative algorithm using
both rapid assays had a sensitivity of 96.9% and a specificity of 99.9%.
The Genie HIV-1/HIV-2 correctly identified 104 of 108 HIV-1 positive
sera, 12 of 13 HIV-2 positive sera, and all six dually positive sera.
Both assays performed well in the field. They required < 10 min to
complete, no equipment, and little training. An algorithm incorporating
two rapid assays can be used as a less expensive alternative to
traditional testing strategies comparable in reliability to ELISA and
Western blot; it provides the additional advantage of differentiating
between HIV-1 and HIV-2.
DE Algorithms Blotting, Western Comparative Study Diagnosis,
Differential Evaluation Studies Human HIV Antibodies/*BLOOD HIV
Infections/*DIAGNOSIS HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY
Immunoenzyme Techniques Nigeria Prostitution Reproducibility of
Results Sensitivity and Specificity JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).